TSPO Antibody | CSB-RA025168A0HU

(No reviews yet) Write a Review
SKU:
CSB-RA025168A0HU
Availability:
3 to 7 Working Days
  • TSPO Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue<br />
 All lanes: PBR antibody at 1.2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 19, 11 KDa<br />
 Observed band size: 19 KDa<br />
  • IHC image of CSB-RA025168A0HU diluted at 1:117 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-RA025168A0HU diluted at 1:117 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of PC3 cells with CSB-RA025168A0HU at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • Immunoprecipitating PTGS2 in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-RA025168A0HU in Hela whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-RA025168A0HU (3µg) + Hela whole cell lysate (500µg)<br />
 Lane 3: Hela whole cell lysate (20µg)<br />
  • Overlay histogram showing HepG2 cells stained with CSB-RA025168A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
€373.00 - €555.00

Description

TSPO Antibody | CSB-RA025168A0HU | Cusabio

TSPO Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant Antibody

Target Names: TSPO

Aliases: Translocator protein, Mitochondrial benzodiazepine receptor, PKBS, Peripheral-type benzodiazepine receptor, PBR, TSPO, BZRP, MBR

Background: Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:24814875), but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides (PubMed:1847678).

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 23G2

Uniport ID: P30536

Modified: Cardiovascular

species: Human

Species Reactivity: Human, Mouse

Immunogen: A synthesized peptide derived from human TSPO

Tested Applications: ELISA, WB, IHC, IF, FC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

View AllClose

0 Reviews

View AllClose