Recombinant Bacillus subtilis Subtilisin E (aprE) | CSB-EP356214BRJ

Recombinant Bacillus subtilis Subtilisin E (aprE) | CSB-EP356214BRJ | Cusabio

Alternative Name(s): aprE; apr; aprA; sprE; BSU10300; Subtilisin E; EC 3.4.21.62

Gene Names: aprE

Research Areas: Others

Organism: Bacillus subtilis (strain 168)

AA Sequence: AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 107-381aa

Sequence Info: Full Length of Mature Protein

MW: 43.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Subtilisin is an Extracellular domain alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.

Reference: "Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation."Stahl M.L., Ferrari E.J. Bacteriol. 158:411-418(1984)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.

Involvement in disease:

Subcellular Location: Secreted

Protein Families: Peptidase S8 family

Tissue Specificity:

Paythway:

Form: Liquid

Buffer: Tris/PBS-based buffer, 5%-50% glycerol

Reconstitution: /

Uniprot ID: P04189

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A