Recombinant Bacillus subtilis Subtilosin-A (sboA) | CSB-EP522484BRJe3
- 3 - 7 Working Days
Recombinant Bacillus subtilis Subtilosin-A (sboA) | CSB-EP522484BRJe3 | Cusabio
Alternative Name(s): Antilisterial bacteriocin subtilosin (sbo)
Gene Names: sboA
Research Areas: Signal Transduction
Organism: Bacillus subtilis (strain 168)
AA Sequence: NKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG
Tag Info: N-terminal 6xHis-KSI-tagged
Expression Region: 9-43aa
Sequence Info: Full Length of Mature Protein
MW: 18.8 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Has bacteriocidal activity against some Gram-positive bacteria such as Listeria, some species of Bacillus and E.faecium, A single mutation (Thr-14-Ile) confers hemolytic activity against rabbit and human blood
Reference: "Subtilosin A, a new antibiotic peptide produced by Bacillus subtilis 168: isolation, structural analysis, and biogenesis." Babasaki K., Takao T., Shimonishi Y., Kurahashi K. J. Biochem. 98:585-603(1985)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Has bacteriocidal activity against some Gram-positive bacteria such as Listeria, some species of Bacillus and E.faecium
Involvement in disease:
Subcellular Location: Secreted
Protein Families: Bacteriocin class V family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: O07623
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A