Recombinant Bacillus subtilis Subtilisin E (aprE) | CSB-EP356214BRJ
- 13 - 23 Working Days
Recombinant Bacillus subtilis Subtilisin E (aprE) | CSB-EP356214BRJ | Cusabio
Alternative Name(s): aprE; apr; aprA; sprE; BSU10300; Subtilisin E; EC 188.8.131.52
Gene Names: aprE
Research Areas: Others
Organism: Bacillus subtilis (strain 168)
AA Sequence: AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 107-381aa
Sequence Info: Full Length of Mature Protein
MW: 43.7 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Subtilisin is an Extracellular domain alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
Reference: "Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation."Stahl M.L., Ferrari E.J. Bacteriol. 158:411-418(1984)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
Involvement in disease:
Subcellular Location: Secreted
Protein Families: Peptidase S8 family
Buffer: Tris/PBS-based buffer, 5%-50% glycerol
Uniprot ID: P04189
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A