KLF4 Antibody | CSB-RA194949A0HU

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SKU:
CSB-RA194949A0HU
Availability:
3 to 7 Working Days
  • KLF4 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, Brain tissue<br />
 All lanes: KLF4 antibody at 1:1500<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 55, 52, 46, 13, 7 kDa<br />
 Observed band size: 55 kDa<br />
  • IHC image of CSB-RA194949A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA194949A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of Hela Cells with CSB-RA194949A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • Immunoprecipitating KLF4 in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-RA194949A0HU in Hela whole cell lysate.
 For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-RA194949A0HU(2µg)+ Hela whole cell lysate(500µg)<br />
 Lane 3: Hela whole cell lysate (10µg)<br />
€373.00 - €555.00

Description

KLF4 Antibody | CSB-RA194949A0HU | Cusabio

KLF4 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Antibody Type: Recombinant

Target Names: KLF4

Aliases: Krueppel-like factor 4 (Epithelial zinc finger protein EZF) (Gut-enriched krueppel-like factor), KLF4, EZF GKLF

Background: Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 3A1

Uniport ID: O43474

Modified: Epigenetics and Nuclear Signaling; Cancer; Cardiovascular; Developmental biology; Stem cells

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human KLF4

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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