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GDNF Antibody | CSB-RA009356A0HU

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CSB-RA009356A0HU
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3 to 7 Working Days
  • GDNF Antibody
  • Western Blot<br />
 Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate<br />
 All lanes: GDNF antibody at 1µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 24, 21, 26, 23, 19 KDa<br />
 Observed band size: 24 KDa<br />
  • IHC image of CSB-RA009356A0HU diluted at 1:107.5 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-RA009356A0HU diluted at 1:107.5 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunoprecipitating GDNF in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-RA009356A0HU in Hela whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-RA009356A0HU (3µg) + Hela whole cell lysate (500µg)<br />
 Lane 3: Hela whole cell lysate (20µg)<br />
  • Overlay histogram showing SH-SY5Y cells stained with CSB-RA009356A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
€373.00 - €555.00
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Description

GDNF Antibody | CSB-RA009356A0HU | Cusabio

GDNF Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant Antibody

Target Names: GDNF

Aliases: Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

Background: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 11D2

Uniport ID: P39905

Modified: Neuroscience

species: Human

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human GDNF

Tested Applications: ELISA, WB, IHC, FC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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