Recombinant Human Dihydrofolate reductase (DHFR), partial | CSB-EP006847HU1
- 13 - 23 Working Days
Recombinant Human Dihydrofolate reductase (DHFR), partial | CSB-EP006847HU1 | Cusabio
Alternative Name(s): DHFR; DHFRP1; Dihydrofolate reductase; DYR; DYR_HUMAN; EC 126.96.36.199
Gene Names: DHFR
Research Areas: Signal Transduction
Organism: Homo sapiens (Human)
AA Sequence: VGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 2-187aa
Sequence Info: Partial
MW: 37.3 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Reference: Identification of a de novo thymidylate biosynthesis pathway in mammalian mitochondria.Anderson D.D., Quintero C.M., Stover P.J.Proc. Natl. Acad. Sci. U.S.A. 108:15163-15168(2011)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Involvement in disease: Megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD)
Subcellular Location: Mitochondrion, Cytoplasm
Protein Families: Dihydrofolate reductase family
Tissue Specificity: Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P00374
HGNC Database Link: HGNC
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: OMIM