Recombinant Human NADPH--cytochrome P450 reductase (POR), partial | CSB-EP018378HU

Recombinant Human NADPH--cytochrome P450 reductase (POR), partial | CSB-EP018378HU | Cusabio

Alternative Name(s): CPR; CYPOR; Cytochrome p450 oxidoreductase; DKFZp686G04235; FLJ26468; NADPH Cytochrome P450 Reductase; NADPH dependent cytochrome P450 reductase; NADPH--cytochrome P450 reductase; NCPR_HUMAN; P450 (cytochrome) oxidoreductase; P450 Cytochrome Oxidoreductase; P450R; por

Gene Names: POR

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: INMGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWLAVCEHFGVEATGEESSIRQYELVVHTDIDAAKVYMGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTALTYYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSWVVEARRHILAILQDCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVVVEYETKAGRINKGVATNWLRAKEPVGENGGRALVPMFVRKSQFRLPFKATTPVIMVGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSDEDYLYREELAQFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGAHIYVCGDARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTK

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 2-671aa

Sequence Info: Partial of BC034277

MW: 102.9 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to he oxygenase and cytochrome B5.

Reference: Mutant P450 oxidoreductase causes disordered steroidogenesis with and without Antley-Bixler syndrome.Flueck C.E., Tajima T., Pandey A.V., Arlt W., Okuhara K., Verge C.F., Jabs E.W., Mendonca B.B., Fujieda K., Miller W.L.Nat. Genet. 36:228-230(2004)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Involvement in disease: Antley-Bixler syndrome, with genital anomalies and disordered steroidogenesis (ABS1); Disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD)

Subcellular Location: Endoplasmic reticulum membrane, Single-pass membrane protein, Cytoplasmic side

Protein Families: NADPH--cytochrome P450 reductase family; Flavodoxin family; Flavoprotein pyridine nucleotide cytochrome reductase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P16435

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM