Cusabio Human Recombinants
Recombinant Human Dihydrofolate reductase (DHFR) | CSB-EP006847HU
- SKU:
- CSB-EP006847HU
- UPC:
- MPN:
- Availability:
- 3 - 7 Working Days
Description
Recombinant Human Dihydrofolate reductase (DHFR) | CSB-EP006847HU | Cusabio
Alternative Name(s): DHFR; DHFRP1; Dihydrofolate reductase; DYR; DYR_HUMAN; EC 1.5.1.3
Gene Names: DHFR
Research Areas: Cell Biology
Organism: Homo sapiens (Human)
AA Sequence: MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
Source: E.coli
Tag Info: N-terminal 6xHis-tagged
Expression Region: 1-187aa
Sequence Info: Full Length
MW: 25.5 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Reference: "Structure-based design and synthesis of lipophilic 2,4-diamino-6-substituted quinazolines and their evaluation as inhibitors of dihydrofolate reductases and potential antitumor agents." Gangjee A., Vidwans A.P., Vasudevan A., Queener S.F., Kisliuk R.L., Cody V., Li R., Galitsky N., Luft J.R., Pangborn W. J. Med. Chem. 41:3426-3434(1998)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Involvement in disease: Megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD)
Subcellular Location: Mitochondrion, Cytoplasm
Protein Families: Dihydrofolate reductase family
Tissue Specificity: Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
Paythway:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P00374
HGNC Database Link: HGNC
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: OMIM
Related Products

Recombinant Human Dihydrofolate reductase (DHFR), partial | CSB-EP006847HU1
Cusabio Human Recombinants

Recombinant Staphylococcus aureus Dihydrofolate reductase (folA) | CSB-EP006847SKY
Cusabio Staphylococcus aureus Recombinants

Recombinant Staphylococcus epidermidis Dihydrofolate reductase (folA) | CSB-EP006847SLD
Cusabio Virus & Bacteria Recombinants

Recombinant Human Methylenetetrahydrofolate reductase (MTHFR) | CSB-EP015158HU
Cusabio Human Recombinants

Recombinant Human Sepiapterin reductase (SPR) | CSB-EP022605HU
Cusabio Human Recombinants
Customers Also Viewed

Recombinant Human Methylenetetrahydrofolate reductase (MTHFR) | CSB-YP015158HU
Cusabio Human Recombinants

Recombinant Human GMP reductase 1 (GMPR) | CSB-EP339756HU
Cusabio Human Recombinants

Recombinant Human Sepiapterin reductase (SPR) | CSB-EP022605HU
Cusabio Human Recombinants

Recombinant Human Methylenetetrahydrofolate reductase (MTHFR) | CSB-EP015158HU
Cusabio Human Recombinants

Recombinant Human Biliverdin reductase A (BLVRA) | CSB-EP002721HU
Cusabio Human Recombinants

E-Tag Monoclonal Antibody | CSB-MA000151M0m
Cusabio Tag & Control

PCNA Monoclonal Antibody | CSB-MA000081M0m
Cusabio Tag & Control

MBP Monoclonal Antibody | CSB-MA000061M0m
Cusabio Tag & Control

GST Monoclonal Antibody | CSB-MA000031M0m
Cusabio Tag & Control

Sumo tag Monoclonal Antibody | CSB-MA000132M0m
Cusabio Tag & Control

GFP Monoclonal Antibody | CSB-MA000051M0m
Cusabio Tag & Control

Myc Mouse Monoclnal Antibody | CSB-MA591321
Cusabio Tag & Control

TUBG1 Monoclonal Antibody | CSB-MA080295
Cusabio Tag & Control

SRT-Tag Monoclonal Antibody | CSB-MA000315
Cusabio Tag & Control

CBP Tag Monoclonal Antibody | CSB-MA000285
Cusabio Tag & Control

Flag-Tag Monoclonal Antibody | CSB-MA000180
Cusabio Tag & Control

Rabbit anti-Mouse IgG Fc Antibody | CSB-PA00580E0Rb
Cusabio Secondary Antibodies

Goat Anti-Rabbit IgG(H+L) Antibody; HRP conjugated | CSB-PA564648
Cusabio Secondary Antibodies

SOX9 Antibody | CSB-RA202969A0HU
Cusabio Recombinant Antibodies

DLG4 Antibody | CSB-RA255792A0HU
Cusabio Recombinant Antibodies

ATM Antibody | CSB-RA166706A0HU
Cusabio Recombinant Antibodies

CD44 Antibody | CSB-RA004938A0HU
Cusabio Recombinant Antibodies

CD146 Antibody | CSB-RA013563A0HU
Cusabio Recombinant Antibodies

OR6C1 Antibody, Biotin conjugated | CSB-PA850422OD01HU
Cusabio Polyclonal Antibodies

OR6C1 Antibody, HRP conjugated | CSB-PA850422OB01HU
Cusabio Polyclonal Antibodies

OR6C1 Antibody | CSB-PA850422OA01HU
Cusabio Polyclonal Antibodies

OR10P1 Antibody, Biotin conjugated | CSB-PA818792OD01HU
Cusabio Polyclonal Antibodies

OR10P1 Antibody, FITC conjugated | CSB-PA818792OC01HU
Cusabio Polyclonal Antibodies

OR10P1 Antibody, HRP conjugated | CSB-PA818792OB01HU
Cusabio Polyclonal Antibodies

OR10P1 Antibody | CSB-PA818792OA01HU
Cusabio Polyclonal Antibodies

DLG4 Antibody | CSB-PA006938LA01HU
Cusabio Polyclonal Antibodies

CD163 Antibody, HRP conjugated | CSB-PA801238LB01HU
Cusabio Polyclonal Antibodies

CD163 Antibody | CSB-PA801238LA01HU
Cusabio Polyclonal Antibodies

FKTN Antibody | CSB-PA008709LA01HU
Cusabio Polyclonal Antibodies

MPN_083 Antibody, Biotin conjugated | CSB-PA303444LD01Mlw
Cusabio Polyclonal Antibodies

MPN_083 Antibody, FITC conjugated | CSB-PA303444LC01Mlw
Cusabio Polyclonal Antibodies

NAAA Antibody | CSB-PA22919A0Rb
Cusabio Polyclonal Antibodies

LPP Antibody, FITC conjugated | CSB-PA856611LC01HU
Cusabio Polyclonal Antibodies

LPP Antibody, HRP conjugated | CSB-PA856611LB01HU
Cusabio Polyclonal Antibodies

WAS Antibody, HRP conjugated | CSB-PA025967LB01HU
Cusabio Polyclonal Antibodies

Fuca1 Antibody, Biotin conjugated | CSB-PA858759LD01MO
Cusabio Polyclonal Antibodies

Fuca1 Antibody, FITC conjugated | CSB-PA858759LC01MO
Cusabio Polyclonal Antibodies

Fuca1 Antibody, HRP conjugated | CSB-PA858759LB01MO
Cusabio Polyclonal Antibodies

dnaK Antibody, Biotin conjugated | CSB-PA633459HD01EGW
Cusabio Polyclonal Antibodies

dnaK Antibody, FITC conjugated | CSB-PA633459HC01EGW
Cusabio Polyclonal Antibodies

dnaK Antibody | CSB-PA633459HA01EGW
Cusabio Polyclonal Antibodies

Cationic trypsin Antibody, HRP conjugated | CSB-PA361971LB01DO
Cusabio Polyclonal Antibodies

Anionic trypsin Antibody, FITC conjugated | CSB-PA356954LC01DO
Cusabio Polyclonal Antibodies

Pollen allergen Phl p 5b Antibody, FITC conjugated | CSB-PA671435HC01EUQ
Cusabio Polyclonal Antibodies

Pollen allergen Phl p 5b Antibody, HRP conjugated | CSB-PA671435HB01EUQ
Cusabio Polyclonal Antibodies