Cusabio Human Recombinants
Recombinant Human Cytochrome c oxidase subunit 1 (MT-CO1), partial | CSB-EP015072HU
- 13 - 23 Working Days
Recombinant Human Cytochrome c oxidase subunit 1 (MT-CO1), partial | CSB-EP015072HU | Cusabio
Alternative Name(s): Cytochrome c oxidase polypeptide I (COI) (COXI) (MTCO1)
Gene Names: MT-CO1
Research Areas: Others
Organism: Homo sapiens (Human)
AA Sequence: EAFASKRKVLMVEEPSMNLEWLYGCPPPYHTFEEPVYMKS
Tag Info: N-terminal 6xHis-GST-tagged
Expression Region: 474-513aa
Sequence Info: Partial
MW: 36.3 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
Reference: "Lineage-specific selection in human mtDNA: lack of polymorphisms in a segment of MTND5 gene in haplogroup J." Moilanen J.S., Finnila S., Majamaa K. Mol. Biol. Evol. 20:2132-2142(2003)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Involvement in disease:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P00395
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A