Recombinant Human CD40 ligand (CD40LG), partial (Active) | CSB-MP004937HU3

Recombinant Human CD40 ligand (CD40LG) ,partial (Active) | CSB-MP004937HU3 | Cusabio

Protein Description: Partial

Alternative Name (s) : (CD40-L) (T-cell antigen Gp39) (NF-related activation protein) (TRAP) (Tumor necrosis factor ligand superfamily member 5) (CD154) (sCD40L)

Gene Names: CD40LG

Research Areas: Cancer

Species: Homo sapiens (Human)

Source: Mammalian cell

Tag Info: N-terminal hFc-Flag-tagged

Expression Region: 13-261aa

Sequence Info: MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Biological Activity: ①Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody（CSB-RA156386A0HU）, the EC50 is 2.353-3.232 ng/ml.

MW: 44.5 kDa

Purity: Greater than 87% as determined by SDS-PAGE.

Endotoxin: Less than 1.0 EU/ug as determined by LAL method.

Relevance: Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973) . Costimulates T-cell proliferation and cytokine production (PubMed:8617933) . Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933) . Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973) . Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037) . Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037) . Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity) . Involved in immunoglobulin class switching

PubMed ID:

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P29965

Uniprot Entry Name:

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A