Recombinant Human CD40 ligand (CD40LG), partial | CSB-YP004937HU1
- 25 - 35 Working Days
Recombinant Human CD40 ligand (CD40LG), partial | CSB-YP004937HU1 | Cusabio
Alternative Name(s): T-cell antigen Gp39 TNF-related activation protein Short name:TRAP Tumor necrosis factor ligand superfamily member 5 CD_antigen: CD154 Cleaved into the following 2 chains: CD40 ligand, membrane form CD40 ligand, soluble form
Gene Names: CD40LG
Research Areas: Immunology
Organism: Homo sapiens (Human)
AA Sequence: HRRLDKIEDERNLHEDFVFMKTIQRCNTGERSLSLLNCEEIKSQFEGFVKDIMLNKEETKKENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
Tag Info: N-terminal 6xHis-tagged
Expression Region: 47-261aa
Sequence Info: Extracellular Domain
MW: 26.1 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Reference: "The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity."Hollenbaugh D., Grosmaire L.S., Kullas C.D., Chalupny J.N., Braesch-Andersen S., Noelle R.J., Stamenkovic I., Ledbetter J.A., Aruffo A.EMBO J. 11:4313-4321(1992)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Cytokine that binds to CD40/TNFRSF5
Involvement in disease: Immunodeficiency with hyper-IgM, type 1 (HIGM1)
Subcellular Location: Cell membrane, Single-pass type II membrane protein, Cell surface, SUBCELLULAR LOCATION: CD40 ligand, soluble form: Secreted
Protein Families: Tumor necrosis factor family
Tissue Specificity: Specifically expressed on activated CD4+ T-lymphocytes.
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P29965
HGNC Database Link: HGNC
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: OMIM