Recombinant Human CD40 ligand (CD40LG), partial (Active) | CSB-AP002211HU

Recombinant Human CD40 ligand (CD40LG) ,partial (Active) | CSB-AP002211HU | Cusabio

Protein Description: Partial

Alternative Name (s) : CD40-L, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD antigen CD154

Gene Names: CD40LG,CD40L,TNFSF5,TRAP

Research Areas: Cancer

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 113-261aa (C194W)

Sequence Info: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.

MW: 16.3 kDa

Purity: >95% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. {ECO:0000269|PubMed:15193700}.; Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. {ECO:0000269|PubMed:15193700}.

PubMed ID: 1280226; 1385114; 7678782; 1281209; 7678552; 7999797; 15489334; 8626375; 11676606; 15193700; 8589998; 9605317; 7679206; 8094231; 7679801; 7717401; 7532185; 8889581; 8550833; 9150729; 9746782

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Cytokine that binds to CD40/TNFRSF5

Involvement in disease: Immunodeficiency with hyper-IgM, type 1 (HIGM1)

Subcellular Location: Cell membrane, Single-pass type II membrane protein, Cell surface, SUBCELLULAR LOCATION: CD40 ligand, soluble form: Secreted

Protein Families: Tumor necrosis factor family

Tissue Specificity: Specifically expressed on activated CD4+ T-lymphocytes.

Paythway: NF-kappaBsignalingpathway

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered PBS, pH 7.0

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P29965

Uniprot Entry Name: CD40L_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM