Recombinant Canine distemper virus Nucleoprotein (N), partial | CSB-YP361393CCQ

Recombinant Canine distemper virus Nucleoprotein (N), partial | CSB-YP361393CCQ | Cusabio

Alternative Name(s): Nucleocapsid protein

Gene Names: N

Research Areas: Microbiology

Organism: Canine distemper virus (strain Onderstepoort) (CDV)

AA Sequence: SKTTEDRTIRATGPKQSQITFLHSERSEVANQQPPTINKRSENQGGDKYPIHFSDERLPGYTPDVNSSEWSESRYDTQIIQDDGNDDDRKSMEAIAKMRMLTKMLSQPGTSEDNSPVYSDKELLN

Source: Yeast

Tag Info: C-terminal 6xHis-Myc-tagged

Expression Region: 399-523aa

Sequence Info: Partial

MW: 17.9 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Encapsidates the genome in a ratio of 1 N per 6 ribonucleotides, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).

Reference: "Canine distemper terminal and intergenic non-protein coding nucleotide sequences: completion of the entire CDV genome sequence." Sidhu M.S., Husar W., Cook S.D., Dowling P.C., Udem S.A. Virology 193:66-72(1993)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P04865

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A