Recombinant Canine distemper virus Nucleoprotein (N), partial | CSB-YP361393CCQ
- 25 - 35 Working Days
Recombinant Canine distemper virus Nucleoprotein (N), partial | CSB-YP361393CCQ | Cusabio
Alternative Name(s): Nucleocapsid protein
Gene Names: N
Research Areas: Microbiology
Organism: Canine distemper virus (strain Onderstepoort) (CDV)
AA Sequence: SKTTEDRTIRATGPKQSQITFLHSERSEVANQQPPTINKRSENQGGDKYPIHFSDERLPGYTPDVNSSEWSESRYDTQIIQDDGNDDDRKSMEAIAKMRMLTKMLSQPGTSEDNSPVYSDKELLN
Tag Info: C-terminal 6xHis-Myc-tagged
Expression Region: 399-523aa
Sequence Info: Partial
MW: 17.9 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Encapsidates the genome in a ratio of 1 N per 6 ribonucleotides, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).
Reference: "Canine distemper terminal and intergenic non-protein coding nucleotide sequences: completion of the entire CDV genome sequence." Sidhu M.S., Husar W., Cook S.D., Dowling P.C., Udem S.A. Virology 193:66-72(1993)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Involvement in disease:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P04865
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A