Recombinant Lassa virus Nucleoprotein (N) | CSB-BP356406LCP

Recombinant Lassa virus Nucleoprotein (N) | CSB-BP356406LCP | Cusabio

Alternative Name(s): Nucleocapsid protein Protein N

Gene Names: N

Research Areas: Signal Transduction

Organism: Lassa virus (strain GA391) (LASV)

AA Sequence: MSASKEVRSCLWTQSLRRELSGYCSNIKLQVVKDAQALLHGLDFSEVSNVQRLMRKQKRDDGDLKRLRDLNQAVNNLVELKSTQQKSVLRVGTLSSDDLLILAADLEKLKSKVTRTERPLSSGVYMGNLSSQQLDQRRALLNMIGMTGVSGGGKGASNGIVRVWDVKNAELLNNQFGTMPSLTLACLTKQGQVDLNDAVQALTDLGLIYTAKYPNSSDLDRLSQSHPILNMIDTKKSSLNISGYNFSLGAAVKAGACMLDGGNMLETIKVSPQTMDGILKSILKVKKSLGMFVSDTPGERNPYENILYKICLSGDGWSYIASRTSIVGRAWENTVVDLEQDNKPQKIGNGGSNKSLQSAGFAAGLIYSQLMTLKDFKCFNLIPNAKTWMDIEGRPEDPVEIALYQPSSGCYVHFFREPTDLKQFKQDAKYSHGRDVTDLFAAQPGLTSAVIEALPRNMVITCQGSEDIRKLLESQGRRDIKLIDITLSKADSRKFENAVWDQFKDLCHMHTGVVVEKKKRGGKEEITPHCALMDCIMFDAAVSGGLDAKVLRVVLPRDMVFRTSTPKVVL

Source: Baculovirus

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 1-570aa

Sequence Info: Full Length

MW: 66.8 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and thereby participates in the suppression of interferon induction.

Reference: "Nucleotide sequence of the S RNA of Lassa virus (Nigerian strain) and comparative analysis of arenavirus gene products." Clegg J.C.S., Wilson S.M., Oram J.D. Virus Res. 18:151-164(1991)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P04935

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A