Recombinant Bacillus subtilis 4-phosphopantetheinyl transferase ffp (ffp) | CSB-EP861741BRI
- 3 - 7 Working Days
Recombinant Bacillus subtilis 4-phosphopantetheinyl transferase ffp (ffp) | CSB-EP861741BRI | Cusabio
Alternative Name(s): Fengycin synthase-activating enzyme (sfp)
Gene Names: ffp
Research Areas: Others
Organism: Bacillus subtilis
AA Sequence: MKIYGIYMDRPLSQEETDRLMSFVSAEKREKCRRFYHKEDAHRTLLGDVLVRSVISEQYQLNKADIRFSAQEYGKPCIPDLPNAHFNISHSGHWVIGAFDSDPIGVDIEKMKPISLGIAERFFSKNEYSDLLSKHKDEQNDYFYHLWSMKESFIKQEGKGLSLPLDSFSVRLHEDGRVSVELPEHHTPCFIKTYEVDPGYKMAVCAARPDFPEDITMISYEALL
Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression Region: 1-224aa
Sequence Info: Full Length
MW: 31.0 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: May activate the peptidyl carrier protein (PCP) domains of fengycin synthase by transferring the 4'-phosphopantetheinyl moiety of coenzyme A to a serine residue.
Reference: "sfp is required for fengycin synthesis." Shu H.-Y., Liu S.-T. Submitted (OCT-2000)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Involvement in disease:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q9F4F7
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A