Recombinant Yersinia enterocolitica Heat-stable enterotoxin B (ystB) | CSB-EP303368YAQ

Recombinant Yersinia enterocolitica Heat-stable enterotoxin B (ystB) | CSB-EP303368YAQ | Cusabio

Alternative Name(s): Y-STB

Gene Names: ystB

Research Areas: Others

Organism: Yersinia enterocolitica

AA Sequence: EENDDWCCEVCCNPACAGC

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 53-71aa

Sequence Info: Full Length of Mature Protein

MW: 18.1 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Toxin which activates the particulate form of guanylate cyclase and increases cyclic GMP levels within the host intestinal epithelial cells. Could play an important role in pathogenesis.

Reference: "The novel heat-stable enterotoxin subtype gene (ystB) of Yersinia enterocolitica: nucleotide sequence and distribution of the yst genes." Ramamurthy T., Yoshino K., Huang X., Nair G.B., Carniel E., Maruyama T., Fukushima H., Takeda T. Microb. Pathog. 23:189-200(1997)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Toxin which activates the particulate form of guanylate cyclase and increases cyclic GMP levels within the host intestinal epithelial cells. Could play an important role in pathogenesis.

Involvement in disease:

Subcellular Location: Secreted

Protein Families: Heat-stable enterotoxin family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P74977

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A