Cusabio Escherichia coli Recombinants
Recombinant Escherichia coli Heat-stable enterotoxin A2 (sta2) | CSB-EP670621ENL
- 3 - 7 Working Days
Recombinant Escherichia coli Heat-stable enterotoxin A2 (sta2) | CSB-EP670621ENL | Cusabio
Alternative Name(s): STA2
Gene Names: sta2
Research Areas: Others
Organism: Escherichia coli
AA Sequence: NSSNYCCELCCNPACTGCY
Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Expression Region: 54-72aa
Sequence Info: Full Length of Mature Protein
MW: 18.1 kDa
Purity: Greater than 85% as determined by SDS-PAGE.
Relevance: Toxin which activates the particulate form of guanylate cyclase and increases cyclic GMP levels within the host intestinal epithelial cells.
Reference: "Rectification of two Escherichia coli heat-stable enterotoxin allele sequences and lack of biological effect of changing the carboxy-terminal tyrosine to histidine." Guzman-Verduzio L.M., Kupersztoch Y.M. Infect. Immun. 57:645-648(1989)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Involvement in disease:
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q47185
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A