Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar) | CSB-YP321441SOO
- 25 - 35 Working Days
Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar) | CSB-YP321441SOO | Cusabio
Alternative Name(s): Phosphinothricin-resistance protein
Gene Names: bar
Research Areas: Others
Organism: Streptomyces hygroscopicus
AA Sequence: MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQEWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGFWQLDFSLPVPPRPVLPVTEI
Tag Info: N-terminal 6xHis-tagged
Expression Region: 1-183aa
Sequence Info: Full Length
MW: 22.6 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group.
Reference: Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus.Thompson C.J., Movva N.R., Tizard R., Crameri R., Davies J.E., Lauwereys M., Botterman J.EMBO J. 6:2519-2523(1987)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance.
Involvement in disease:
Protein Families: Acetyltransferase family, PAT/BAR subfamily
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P16426
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A