Recombinant Rat Heat-stable enterotoxin receptor (Gucy2c), partial | CSB-MP010053RA

Recombinant Rat Heat-stable enterotoxin receptor (Gucy2c), partial | CSB-MP010053RA | Cusabio

Alternative Name(s): Guanylyl cyclase C (GC-C) (Intestinal guanylate cyclase) (Gucy2c) (Guc2c)

Gene Names: Gucy2c

Research Areas: Microbiology

Organism: Rattus norvegicus (Rat)

AA Sequence: SQVRQKCHNGTYEISVLMMDNSAYKEPLQNLRDAVEEGLDIVRKRLREAELNVTVNATFIYSDGLIHKSGDCRSSTCEGLDLLREITRDRKMGCVLMGPSCTYSTFQMYLDTELNYPMISAGSFGLSCDYKETLTRILPPARKLMYFLVDFWKVNNAPFKTFSWNSSYVYKNGSEPEDCFWYLNALEAGVSYFSEVLSFKDVLRRSEQFQEILMGRNRKSNVIVMCGTPETFYNVKGDLKVADDTVVILVDLFSNHYFEDDTRAPEYMDNVLVLTLPPEKFIANASVSGRFPSERSDFSLAYLEGTLLFGHMLQTFLENGESVTTPKFARAFRNLTFQGLEGPVTLDDSGDIDNIMCLLYVSLDTRKYKVLMAYDTHKNQTIPVATSPNFIWKNHRLPNDVPGLGPQ

Source: Mammalian cell

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 23-429aa

Sequence Info: Extracellular Domain

MW: 51.4 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.

Reference: "Guanylyl cyclase is a heat-stable enterotoxin receptor." Schulz S., Green C.K., Yuen P.S.T., Garbers D.L. Cell 63:941-948(1990)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.

Involvement in disease:

Subcellular Location: Cell membrane, Single-pass type I membrane protein, Endoplasmic reticulum membrane, Single-pass type I membrane protein

Protein Families: Adenylyl cyclase class-4/guanylyl cyclase family

Tissue Specificity: Intestine and possibly at low levels in additional tissues.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P23897

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A