Recombinant Rat Heat-stable enterotoxin receptor (Gucy2c), partial | CSB-YP010053RA
- 25 - 35 Working Days
Recombinant Rat Heat-stable enterotoxin receptor (Gucy2c), partial | CSB-YP010053RA | Cusabio
Alternative Name(s): Guanylyl cyclase C (GC-C) (Intestinal guanylate cyclase Guc2c)
Gene Names: Gucy2c
Research Areas: Microbiology
Organism: Rattus norvegicus (Rat)
AA Sequence: SQVRQKCHNGTYEISVLMMDNSAYKEPLQNLRDAVEEGLDIVRKRLREAELNVTVNATFIYSDGLIHKSGDCRSSTCEGLDLLREITRDRKMGCVLMGPSCTYSTFQMYLDTELNYPMISAGSFGLSCDYKETLTRILPPARKLMYFLVDFWKVNNAPFKTFSWNSSYVYKNGSEPEDCFWYLNALEAGVSYFSEVLSFKDVLRRSEQFQEILMGRNRKSNVIVMCGTPETFYNVKGDLKVADDTVVILVDLFSNHYFEDDTRAPEYMDNVLVLTLPPEKFIANASVSGRFPSERSDFSLAYLEGTLLFGHMLQTFLENGESVTTPKFARAFRNLTFQGLEGPVTLDDSGDIDNIMCLLYVSLDTRKYKVLMAYDTHKNQTIPVATSPNFIWKNHRLPNDVPGLGPQ
Tag Info: N-terminal 6xHis-tagged and C-terminal Myc-tagged
Expression Region: 23-429aa
Sequence Info: Partial
MW: 49.9 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.
Reference: "Guanylyl cyclase is a heat-stable enterotoxin receptor." Schulz S., Green C.K., Yuen P.S.T., Garbers D.L. Cell 63:941-948(1990)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.
Involvement in disease:
Subcellular Location: Cell membrane, Single-pass type I membrane protein, Endoplasmic reticulum membrane, Single-pass type I membrane protein
Protein Families: Adenylyl cyclase class-4/guanylyl cyclase family
Tissue Specificity: Intestine and possibly at low levels in additional tissues.
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P23897
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A