Recombinant Nicotiana tabacum Osmotin (AP24) | CSB-EP320333NHE

Recombinant Nicotiana tabacum Osmotin (AP24) | CSB-EP320333NHE | Cusabio

Alternative Name(s): AP24; Osmotin

Gene Names: AP24

Research Areas: Others

Organism: Nicotiana tabacum (Common tobacco)

AA Sequence: ATIEVRNNCPYTVWAASTPIGGGRRLDRGQTWVINAPRGTKMARVWGRTNCNFNAAGRGTCQTGDCGGVLQCTGWGKPPNTLAEYALDQFSGLDFWDISLVDGFNIPMTFAPTNPSGGKCHAIHCTANINGECPRELRVPGGCNNPCTTFGGQQYCCTQGPCGPTFFSKFFKQRCPDAYSYPQDDPTSTFTCPGGSTNYRVIFCPNGQAHPNFPLEMPGSDEVAK

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 22-246aa

Sequence Info: Full Length of Mature Protein

MW: 40.3 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance:

Reference: Crystal structure of osmotin, a plant antifungal protein.Min K., Ha S.C., Hasegawa P.M., Bressan R.A., Yun D.J., Kim K.K.Proteins 54:170-173(2004)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location: Vacuole

Protein Families: Thaumatin family

Tissue Specificity: Highly expressed in roots and flower buds.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P14170

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A