Recombinant Nicotiana alata Flower-specific defensin (D1) | CSB-EP814081NDF

Recombinant Nicotiana alata Flower-specific defensin (D1) | CSB-EP814081NDF | Cusabio

Alternative Name(s): NaD1

Gene Names: D1

Research Areas: Others

Organism: Nicotiana alata (Winged tobacco) (Persian tobacco)

AA Sequence: RECKTESNTFPGICITKPPCRKACISEKFTDGHCSKILRRCLCTKPC

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 26-72aa

Sequence Info: Full Length of Mature Protein

MW: 21.3 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea. Retards the growth of the Lepidopteran insect pests H.armigera and H.punctigera.

Reference: "The three-dimensional solution structure of NaD1, a new floral defensin from Nicotiana alata and its application to a homology model of the crop defense protein alfAFP." Lay F.T., Schirra H.J., Scanlon M.J., Anderson M.A., Craik D.J. J. Mol. Biol. 325:175-188(2003)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea. Retards the growth of the Lepidopteran insect pests H.armigera and H.punctigera.

Involvement in disease:

Subcellular Location: Secreted, Vacuole

Protein Families: DEFL family

Tissue Specificity: Most abundant in the epidermal cell layers of the petals and sepals, within the connective cells of the anthers, and the cortical cells of the style. Not detected in the tapetum, pollen mother cells, the transmitting tissue, the vascular bundles of the anther and style or in leaves. Expressed also in ovaries, but barley detectable in roots.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q8GTM0

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A