Recombinant Mouse Interleukin-1 beta protein (Il1b) (Active) | CSB-AP003261MO

Recombinant Mouse Interleukin-1 beta protein (Il1b) (Active) | CSB-AP003261MO | Cusabio

Protein Description: Full Length of Mature Protein

Alternative Name (s) : IL-1 beta,

Gene Names: Il1b

Research Areas: Immunology

Species: Mus musculus (Mouse)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 118-269aa

Sequence Info: VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of ＞ 5.0 × 108 IU/mg.

MW: 17.5 kDa

Purity: >96% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

PubMed ID: 3491144; 3492706; 15489334; 2967326; 1807351

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.

Involvement in disease:

Subcellular Location: Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted

Protein Families: IL-1 family

Tissue Specificity: Expressed in activated macrophages (at protein level) .

Paythway:

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P10749

Uniprot Entry Name: IL1B_MOUSE

HGNC Database Link: N/A

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A