Recombinant Mouse Interleukin-1 beta protein (Il1b) (Active) | CSB-AP003261MO
- 5 to 10 Working Days
Recombinant Mouse Interleukin-1 beta protein (Il1b) (Active) | CSB-AP003261MO | Cusabio
Protein Description: Full Length of Mature Protein
Alternative Name (s) : IL-1 beta,
Gene Names: Il1b
Research Areas: Immunology
Species: Mus musculus (Mouse)
Tag Info: Tag-Free
Expression Region: 118-269aa
Sequence Info: VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of ＞ 5.0 × 108 IU/mg.
MW: 17.5 kDa
Purity: >96% as determined by SDS-PAGE and HPLC.
Endotoxin: Less than 1.0 EU/µg as determined by LAL method.
Relevance: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
PubMed ID: 3491144; 3492706; 15489334; 2967326; 1807351
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Function: Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Involvement in disease:
Subcellular Location: Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted
Protein Families: IL-1 family
Tissue Specificity: Expressed in activated macrophages (at protein level) .
Form: Lyophilized powder
Buffer: Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P10749
Uniprot Entry Name: IL1B_MOUSE
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A