Recombinant Lactobacillus reuteri 60KDA chaperonin (groL) | CSB-YP403635LLF

Recombinant Lactobacillus reuteri 60KDA chaperonin (groL) | CSB-YP403635LLF | Cusabio

Alternative Name(s): GroEL protein,Protein Cpn60

Gene Names: groL

Research Areas: Others

Organism: Lactobacillus reuteri (strain DSM 20016)

AA Sequence: MAKEIKFSEDARSAMLSGVDKLADTVKTTMGPKGRNVVLEQSYGTPTITNDGVTIAKSIELENQFENMGAKLVAEVASKTNDIAGDGTTTATVLTQAIVNAGMKNVTSGANPVGIRRGIDKATEVAVEALKKMSHDVKTKDDIEQIASISAANPEVGKLIADAMEKVGHDGVITIEDSRGVDTSVDVVEGMSFDRGYMSQYMVTDNDKMEADLDNPYILITDKKISNIQDILPLLQSVVQEGRALLIIADDITGEALPTLVLNKIRGTFNVVSVKAPGFGDRRKAQLQDIAVLTGGTVISDDLGMSLKDATVDQLGQANKVTVTKDTTTIVDGAGSKEAIQERVDQIKQEIAKSTSDFDKEKLQERLAKLSGGVAVVKVGAATETELKEKKYRIEDALNATRAAVQEGFVPGGGTALINVIPALDKIEADGDELTGINIVKAALEAPVRQIAENAGVEGSVIVNELKNEKEGIGYNAADGKFEDMIKAGIVDPTMVTRSALQNAASVSALLLTTEAVVADKPDPNANNQAPAAPQGGMGGMM

Source: Yeast

Tag Info: N-terminal 6xHis-tagged

Expression Region: 1-542aa

Sequence Info: Full Length

MW: 59.1 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.

Reference: "The evolution of host specialization in the vertebrate gut symbiont Lactobacillus reuteri."Frese S.A., Benson A.K., Tannock G.W., Loach D.M., Kim J., Zhang M., Oh P.L., Heng N.C., Patil P.B., Juge N., Mackenzie D.A., Pearson B.M., Lapidus A., Dalin E., Tice H., Goltsman E., Land M., Hauser L. Walter J.PLoS Genet. 7:E1001314-E1001314(2011)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.

Involvement in disease:

Subcellular Location: Cytoplasm

Protein Families: Chaperonin (HSP60) family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: A5VIE9

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: N/A

OMIM Database Link: N/A