Recombinant Chlamydia trachomatis 60KDA chaperonin (groL) | CSB-EP314770DSB
- 3 - 7 Working Days
Recombinant Chlamydia trachomatis 60KDA chaperonin (groL) | CSB-EP314770DSB | Cusabio
Alternative Name(s): 57KDA chlamydial hypersensitivity antigen GroEL protein Heat shock protein 60 HSP60 Protein Cpn60
Gene Names: groL
Research Areas: Microbiology
Organism: Chlamydia trachomatis (strain D/UW-3/Cx)
AA Sequence: VAKNIKYNEEARKKIQKGVKTLAEAVKVTLGPKGRHVVIDKSFGSPQVTKDGVTVAKEVELADKHENMGAQMVKEVASKTADKAGDGTTTATVLAEAIYTEGLRNVTAGANPMDLKRGIDKAVKVVVDQIRKISKPVQHHKEIAQVATISANNDAEIGNLIAEAMEKVGKNGSITVEEAKGFETVLDIVEGMNFNRGYLSSYFATNPETQECVLEDALVLIYDKKISGIKDFLPVLQQVAESGRPLLIIAEDIEGEALATLVVNRIRGGFRVCAVKAPGFGDRRKAMLEDIAILTGGQLISEELGMKLENANLAMLGKAKKVIVSKEDTTIVEGMGEKEALEARCESIKKQIEDSSSDYDKEKLQERLAKLSGGVAVIRVGAATEIEMKEKKDRVDDAQHATIAAVEEGILPGGGTALIRCIPTLEAFLPMLTNEDEQIGARIVLKALSAPLKQIAANAGKEGAIIFQQVMSRSANEGYDALRDAYTDMLEAGILDPAKVTRSALESAASVAGLLLTTEALIAEIPEEKPAAAPAMPGAGMDY
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 2-544aa
Sequence Info: Full Length of Mature Protein
MW: 74.0 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity). This protein is implicated in the pathogenesis of chlamydial disease. Inflammation elicited by the 57KDA antigen may damage tissue, with progression to scarring of conjunctival and fallopian tube mucosae, which respectively result in blindness and infertility.
Reference: "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.Science 282:754-759(1998)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity). This protein is implicated in the pathogenesis of chlamydial disease. Inflammation elicited by the 57 kDa antigen may damage tissue, with progression to scarring of conjunctival and fallopian tube mucosae, which respectively result in blindness and infertility.
Involvement in disease:
Subcellular Location: Cytoplasm
Protein Families: Chaperonin (HSP60) family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P0C0Z7
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: KEGG
STRING Database Link: N/A
OMIM Database Link: N/A