Recombinant Junin mammarenavirus Nucleoprotein (N) | CSB-EP318492JAM

Recombinant Junin mammarenavirus Nucleoprotein (N) | CSB-EP318492JAM | Cusabio

Alternative Name(s): Nucleocapsid protein Protein N

Gene Names: N

Research Areas: Others

Organism: Junin mammarenavirus (JUNV) (Junn mammarenavirus)

AA Sequence: MAHSKEVPSFRWTQSLRRGLSQFTQTVKSDVLKDAKLIADSIDFNQVAQVQRALRKTKRGEEDLNKLRDLNKEVDRLMSMRSVQRNTVFKAGDLGRVERMELASGLGNLKTKFRRAETGSQGVYMGNLSQSQLAKRSEILRTLGFQQQGTGGNGVVRVWDVKDPSKLNNQFGSVPALTIACMTVQGGETMNSVIQALTSLGLLYTVKYPNLSDLDRLTQEHDCLQIVTKDESSINISGYNFSLSAAVKAGASILDDGNMLETIRVTPDNFSSLIKSTIQVKRREGMFIDEKPGNRNPYENLLYKLCLSGDGWPYIGSRSQIIGRSWDNTSIDLTRKPVAGPRQPEKNGQNLRLANLTEIQEAVIREAVGKLDPTNTLWLDIEGPATDPVEMALFQPAGSKYIHCFRKPHDEKGFKNGSRHSHGILMKDIEDAMPGVLSYVIGLLPPDMVVTTQGSDDIRKLFDLHGRRDLKLVDVRLTSEQARQFDQQVWEKFGHLCKHHNGVVVSKKKRDKDAPFKLASSEPHCALLDCIMFQSVLDGKLYEEELTPLLPPSLLFLPKAAYAL

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-564aa

Sequence Info: Full Length

MW: 79 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3 phosphorylation and activation by host IKBKE. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of IRF3, a protein involved in IFN activation pathway, leading to the inhibition of IFNB and IRF3-dependent promoters activation (By similarity). Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.

Reference: "Molecular organization of Junin virus S RNA: complete nucleotide sequence, relationship with other members of the Arenaviridae and unusual secondary structures."Ghiringhelli P.D., Rivera-Pomar R.V., Lozano M.E., Grau O., Romanowski V.J. Gen. Virol. 72:2129-2141(1991)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3 phosphorylation and activation by host IKBKE. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of IRF3, a protein involved in IFN activation pathway, leading to the inhibition of IFNB and IRF3-dependent promoters activation (By similarity). Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.

Involvement in disease:

Subcellular Location: Virion, Host cytoplasm

Protein Families: Arenaviridae nucleocapsid protein family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P14239

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A