Recombinant Human Sonic hedgehog protein (SHH) (Active) | CSB-AP000371HU

Recombinant Human Sonic hedgehog protein (SHH) (Active) | CSB-AP000371HU | Cusabio

Protein Description: Partial

Alternative Name (s) : SHH,

Gene Names: SHH

Research Areas: Cancer

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 25-197aa

Sequence Info: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of ＞ 1.0 × 103 IU/mg.

MW: 19.8 kDa

Purity: >98% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity) . {ECO:0000250}.

PubMed ID: 7590746; 12853948; 12690205; 9593755; 7720571; 12837695; 16335952; 18417549; 19847792; 24456159; 24965254; 10753901; 19561609; 20504762; 8896572; 9302262; 10441331; 10556296; 11471164; 11479728; 12503095; 15103725; 15107988; 15221788; 15942952; 15942953; 16282375; 17001669; 19603532

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Sonic hedgehog protein

Involvement in disease: Microphthalmia, isolated, with coloboma, 5 (MCOPCB5) ; Holoprosencephaly 3 (HPE3) ; Solitary median maxillary central incisor (SMMCI) ; Triphalangeal thumb-polysyndactyly syndrome (TPTPS) ; Preaxial polydactyly 2 (PPD2) ; Hypoplasia or aplasia of tibia with polydactyly (THYP) ; Laurin-Sandrow syndrome (LSS)

Subcellular Location: Sonic hedgehog protein N-product: Cell membrane, Lipid-anchor

Protein Families: Hedgehog family

Tissue Specificity:

Paythway: Hedgehogsignalingpathway

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q15465

Uniprot Entry Name: SHH_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM