Recombinant Human Sialidase-3 (NEU3), partial | CSB-EP891986HU

Recombinant Human Sialidase-3 (NEU3), partial | CSB-EP891986HU | Cusabio

Alternative Name(s): Ganglioside sialidase;Membrane sialidaseN-acetyl-alpha-neuraminidase 3

Gene Names: NEU3

Research Areas: Metabolism

Organism: Homo sapiens (Human)

AA Sequence: EEVTTCSFNSPLFRQEDDRGITYRIPALLYIPPTHTFLAFAEKRSTRRDEDALHLVLRRGLRIGQLVQWGPLKPLMEATLPGHRTMNPCPVWEQKSGCVFLFFICVRGHVTERQQIVSGRNAARLCFIYSQDAGCSWSEVRDLTEEVIGSELKHWATFAVGPGHGIQLQSGRLVIPAYTYYIPSWFFCFQLPCKTRPHSLMIYSDDLGVTWHHGRLIRPMVTVECEVAEVTGRAGHPVLYCSARTPNRCRAEALSTDHGEGFQRLALSRQLCEPPHGCQGSVVSFRPLEIPHRCQDSSSKDAPTIQQSSPGSSLRLEEEAGTPSESWLLYSHPTSRKQRVDLGIYLNQTPLEAACWSRPWILHCGPCGYSDLAALEEEGLFGCLFECGTKQECEQIAFRLFTHREILSHLQGDCTSPGRNPSQFKSN

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 2-428aa

Sequence Info: Partial

MW: 52.1 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Plays a role in modulating the ganglioside content of the lipid bilayer at the level of mbrane-bound sialyl glycoconjugates.

Reference: Cloning, expression, and chromosomal mapping of a human ganglioside sialidase.Wada T., Yoshikawa Y., Tokuyama S., Kuwabara M., Akita H., Miyagi T.Biochem. Biophys. Res. Commun. 261:21-27(1999)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates.

Involvement in disease:

Subcellular Location: Cell membrane, Peripheral membrane protein

Protein Families: Glycosyl hydrolase 33 family

Tissue Specificity: Highly expressed in skeletal muscle, testis, adrenal gland and thymus, followed by pancreas, liver, heart and thymus. Weakly expressed in kidney, placenta, brain and lung.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9UQ49

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM