Recombinant Human Programmed cell death protein 1 (PDCD1), partial (Active) | CSB-MP619964HU1
- 3 to 7 Working Days
Recombinant Human Programmed cell death protein 1 (PDCD1) ,partial (Active) | CSB-MP619964HU1 | Cusabio
Protein Description: Partial
Alternative Name (s) : (Protein PD-1) (hPD-1) (CD279)
Gene Names: PDCD1
Research Areas: Cancer
Species: Homo sapiens (Human)
Source: Mammalian cell
Tag Info: C-terminal 6xHis-tagged
Expression Region: 25-167aa
Sequence Info: LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ
Biological Activity: ①Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Anti-PD-1 recombinant antibody, the EC50 of human PD-1 protein is 6.087-7.854 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Nivolumab, the EC50 of human PD-1 protein is 9.713-12.39 ng/ml.
MW: 20.0 kDa
Purity: Greater than 93% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
Relevance: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Involvement in disease:
Form: Lyophilized powder
Buffer: Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q15116
Uniprot Entry Name:
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A