Recombinant Human Phytanoyl-CoA dioxygenase, peroxisomal (PHYH) | CSB-EP017946HU
- 13 - 23 Working Days
Recombinant Human Phytanoyl-CoA dioxygenase, peroxisomal (PHYH) | CSB-EP017946HU | Cusabio
Alternative Name(s): Phytanic acid oxidase Phytanoyl-CoA alpha-hydroxylase
Gene Names: PHYH
Research Areas: Signal Transduction
Organism: Homo sapiens (Human)
AA Sequence: SGTISSASFHPQQFQYTLDNNVLTLEQRKFYEENGFLVIKNLVPDADIQRFRNEFEKICRKEVKPLGLTVMRDVTISKSEYAPSEKMITKVQDFQEDKELFRYCTLPEILKYVECFTGPNIMAMHTMLINKPPDSGKKTSRHPLHQDLHYFPFRPSDLIVCAWTAMEHISRNNGCLVVLPGTHKGSLKPHDYPKWEGGVNKMFHGIQDYEENKARVHLVMEKGDTVFFHPLLIHGSGQNKTQGFRKAISCHFASADCHYIDVKGTSQENIEKEVVGIAHKFFGAENSVNLKDIWMFRARLVKGERTNL
Tag Info: N-terminal GST-tagged
Expression Region: 1-338aa
Sequence Info: Full Length
MW: 62.4 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA.
Reference: "Identification of PAHX, a Refsum disease gene." Mihalik S.J., Morrell J.C., Kim D., Sachsteder K.A., Watkins P.A., Gould S.J. Nat. Genet. 17:185-189(1997)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA.
Involvement in disease: Refsum disease (RD)
Subcellular Location: Peroxisome
Protein Families: PhyH family
Tissue Specificity: Expressed in liver, kidney, and T-cells, but not in spleen, brain, heart, lung and skeletal muscle.
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: O14832
HGNC Database Link: HGNC
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: OMIM