Recombinant Human Nectin-4 (NECTIN4), partial (Active) | CSB-AP005571HU

Recombinant Human Nectin-4 (NECTIN4) ,partial (Active) | CSB-AP005571HU | Cusabio

Protein Description: Partial

Alternative Name (s) : PVRL4;Nectin-4;Ig superfamily receptor LNIR;Poliovirus receptor-related protein 4;PRR4;LNIR

Gene Names: NECTIN4

Research Areas: Cell Biology

Species: Homo sapiens (Human)

Source: Mammalian cell

Tag Info: C-terminal 6xHis-tagged

Expression Region: 32-351aa

Sequence Info: GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASVV

Biological Activity: Specific activity as determined by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells is greater than 40%. When 5 x 10^4 cells/well are added to plates coated with recombinant human Nectin-4 (1 µg/mL with 100 µL/well) , approximately >50% will adhere after 40 min incubation at 37 °C.

MW: 35.3 kDa

Purity: Greater than 95% as determined by SDS-PAGE.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding) , probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.

PubMed ID:

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.; FUNCTION

Involvement in disease: Ectodermal dysplasia-syndactyly syndrome 1 (EDSS1)

Subcellular Location: Cell membrane, Single-pass type I membrane protein, Cell junction, adherens junction, Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254) , SUBCELLULAR LOCATION: Processed poliovirus receptor-related protein 4: Secreted

Protein Families: Nectin family

Tissue Specificity: Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.

Paythway: Adherensjunction

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q96NY8

Uniprot Entry Name:

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM