Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor protein (MANF) (Active) | CSB-AP002891HU

Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor protein (MANF) (Active) | CSB-AP002891HU | Cusabio

Protein Description: Full Length of Mature Protein

Alternative Name (s) : Arginine-rich protein,

Gene Names: MANF,ARMET,ARP

Research Areas: Neuroscience

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 25-182aa

Sequence Info: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of ＞ 50 IU/mg.

MW: 18.2 kDa

Purity: >95% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity) . Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. {ECO:0000250, ECO:0000269|PubMed:12794311, ECO:0000269|PubMed:18561914}.

PubMed ID: 8649854; 15489334; 16641997; 12665801; 12794311; 15144186; 18561914; 21269460; 24275569; 19258449; 8971156; 9174057

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity) . Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death.

Involvement in disease:

Subcellular Location: Secreted

Protein Families: ARMET family

Tissue Specificity:

Paythway:

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P55145

Uniprot Entry Name: MANF_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM