Recombinant Human Malate dehydrogenase, cytoplasmic (MDH1) (Active) | CSB-AP005651HU
- 5 to 10 Working Days
Recombinant Human Malate dehydrogenase, cytoplasmic (MDH1) (Active) | CSB-AP005651HU | Cusabio
Protein Description: Full Length of Mature Protein
Alternative Name (s) : Malate Dehydrogenase Cytoplasmic; Cytosolic Malate Dehydrogenase; Diiodophenylpyruvate Reductase; MDH1; MDHA
Gene Names: MDH1
Research Areas: Signal Transduction
Species: Homo sapiens (Human)
Tag Info: C-terminal 6xHis-tagged
Expression Region: 2-334aa
Sequence Info: SEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA
Biological Activity: Its dehydrogenation activity from (S) -malate to oxaloacetate in the presense of NAD+ is determined to be greater than 1000 pmol/min/ug
MW: 37.5 kDa
Purity: Greater than 95% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU/µg as determined by LAL method.
Relevance: Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Pxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner Mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Involvement in disease:
Subcellular Location: Cytoplasm
Protein Families: LDH/MDH superfamily, MDH type 2 family
Buffer: 0.2 μm filtered 20 mM Tris-HCl, 150 mM NaCl, pH 8.0
Uniprot ID: P40925
Uniprot Entry Name:
HGNC Database Link: HGNC
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: OMIM