Cusabio Escherichia coli Recombinants
Recombinant Escherichia coli Malate dehydrogenase (mdh) | CSB-EP013623ENV
- 13 - 23 Working Days
Recombinant Escherichia coli Malate dehydrogenase (mdh) | CSB-EP013623ENV | Cusabio
Alternative Name(s): mdh; b3236; JW3205Malate dehydrogenase; EC 220.127.116.11
Gene Names: mdh
Research Areas: Others
Organism: Escherichia coli (strain K12)
AA Sequence: MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAVDLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLVQQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAELKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVEERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK
Tag Info: N-terminal 6xHis-tagged
Expression Region: 1-312aa
Sequence Info: Full Length
MW: 36.3 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Catalyzes the reversible oxidation of malate to oxaloacetate.
Reference: Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase.Sutherland P., McAlister-Henn L.J. Bacteriol. 163:1074-1079(1985)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Catalyzes the reversible oxidation of malate to oxaloacetate.
Involvement in disease:
Protein Families: LDH/MDH superfamily, MDH type 1 family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: P61889
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A