Recombinant Human Lipoyltransferase 1, mitochondrial (LIPT1) | CSB-EP896479HU

Recombinant Human Lipoyltransferase 1, mitochondrial (LIPT1) | CSB-EP896479HU | Cusabio

Alternative Name(s): Lipoate biosynthesis protein Lipoate-protein ligase Lipoyl ligase

Gene Names: LIPT1

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: KTVKNGLILQSISNDVYQNLAVEDWIHDHMNLEGKPILFFWQNSPSVVIGRHQNPWQECNLNLMREEGIKLARRRSGGGTVYHDMGNINLTFFTTKKKYDRMENLKLIVRALNAVQPQLDVQATKRFDLLLDGQFKISGTASKIGRTTAYHHCTLLCSTDGTFLSSLLKSPYQGIRSNATASIPSLVKNLLEKDPTLTCEVLMNAVATEYAAYHQIDNHIHLINPTDETLFPGINSKAKELQTWEWIYGKTPKFSINTSFHVLYEQSHLEIKVFIDIKNGRIEICNIEAPDHWLPLEIRDKLNSSLIGSKFCPTETTMLTNILLRTCPQDHKLNSKWNILCEKIKGIM

Source: E.coli

Tag Info: N-terminal 6xHis-tagged

Expression Region: 26-373aa

Sequence Info: Full Length of Mature Protein

MW: 45.7 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Relevance: Catalyzes the transfer of the lipoyl group from lipoyl-AMP to the specific lysine residue of lipoyl domains of lipoate-dependent enzymes.

Reference: "Mutations in human lipoyltransferase gene LIPT1 cause a Leigh disease with secondary deficiency for pyruvate and alpha-ketoglutarate dehydrogenase." Soreze Y., Boutron A., Habarou F., Barnerias C., Nonnenmacher L., Delpech H., Mamoune A., Chretien D., Hubert L., Bole-Feysot C., Nitschke P., Correia I., Sardet C., Boddaert N., Hamel Y., Delahodde A., Ottolenghi C., de Lonlay P. Orphanet J. Rare Dis. 8:192-192(2013)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:

Involvement in disease:

Subcellular Location:

Protein Families:

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q9Y234

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: N/A

STRING Database Link: N/A

OMIM Database Link: N/A