Recombinant Human Leukocyte surface antigen CD47 (CD47), partial (Active) | CSB-MP004940HU
- 3 to 7 Working Days
Recombinant Human Leukocyte surface antigen CD47 (CD47) ,partial (Active) | CSB-MP004940HU | Cusabio
Protein Description: Partial
Alternative Name (s) : Antigenic surface determinant protein OA3（Integrin-associated protein）（IAP）（Protein MER6）（CD47）
Gene Names: CD47
Research Areas: Cancer
Species: Homo sapiens (Human)
Source: Mammalian cell
Tag Info: C-terminal hFc-tagged
Expression Region: 19-139aa
Sequence Info: QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP
Biological Activity: ①Measured by its binding ability in a functional ELISA. Immobilized SIRPA (CSB-MP021334HU) at 2 μg/ml can bind human CD47, the EC50 of human CD47 protein is 65.91-82.42 ng/ml. ②Human SIRPA protein His/Myc tag (CSB-MP021334HU) captured on COOH chip can bind Human CD47 protein Fc tag (CSB-MP004940HU) with an affinity constant of 19.1 nM as detected by LSPR Assay.
MW: 41.5 kDa
Purity: Greater than 95% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
Relevance: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Involvement in disease:
Form: Lyophilized powder
Buffer: Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q08722
Uniprot Entry Name:
HGNC Database Link: N/A
UniGene Database Link: N/A
KEGG Database Link: N/A
STRING Database Link: N/A
OMIM Database Link: N/A