Recombinant Human Interleukin-1 receptor antagonist protein (IL1RN) (Active) | CSB-AP001681HU

Recombinant Human Interleukin-1 receptor antagonist protein (IL1RN) (Active) | CSB-AP001681HU | Cusabio

Protein Description: Full Length of Mature Protein

Alternative Name (s) : IL-1RN, IRAP, ICIL-1RA, IL1 inhibitor, Anakinra

Gene Names: IL1RN,IL1F3,IL1RA

Research Areas: Immunology

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 26-177aa

Sequence Info: RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α- dependent proliferation of murine D10S cells is less than 40 ng/ml, corresponding to a specific activity of ＞ 2.5 × 104 IU/mg in the presence of 50 pg/ml rHuIL-1α.

MW: 17.1 kDa

Purity: >96% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. {ECO:0000269|PubMed:7775431}.

PubMed ID: 2139180; 2137201; 1828896; 1385987; 8992991; 1827201; 7629520; 14702039; 15815621; 15489334; 2137200; 2143761; 9514884; 7989776; 7775431; 11991722; 8786086; 16335952; 19494218; 1534997; 8045306; 8175703; 7867645; 9062194; 18987736

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.

Involvement in disease: Microvascular complications of diabetes 4 (MVCD4) ; Interleukin 1 receptor antagonist deficiency (DIRA)

Subcellular Location: Isoform 1: Secreted, SUBCELLULAR LOCATION: Isoform 2: Cytoplasm, SUBCELLULAR LOCATION: Isoform 3: Cytoplasm, SUBCELLULAR LOCATION: Isoform 4: Cytoplasm

Protein Families:

Tissue Specificity: The intracellular form of IL1RN is predominantly expressed in epithelial cells.

Paythway:

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P18510

Uniprot Entry Name: IL1RA_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM