Recombinant Human Insulin-like growth factor II protein (IGF2) (Active) | CSB-AP002551HU

Recombinant Human Insulin-like growth factor II protein (IGF2) (Active) | CSB-AP002551HU | Cusabio

Protein Description: Partial

Alternative Name (s) : IGF-II, T3M-11-derived growth factor,

Gene Names: IGF2,PP1446

Research Areas: Cancer

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 25-91aa

Sequence Info: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of ＞ 5.0 × 105 IU/mg.

MW: 7.5 kDa

Purity: >98% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.; Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

PubMed ID: 6382022; 6382021; 2450353; 3002851; 3476948; 3683205; 3881277; 7730145; 16531418; 20842449; 15498874; 14702039; 16554811; 15489334; 3167054; 3653397; 3652904; 658418; 2722836; 7633596; 11448941; 12586351; 15359740; 1569071; 16912056; 19066168; 19838169; 22171320; 23234360; 6189745; 7527339; 18046459

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity) . In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable) .

Involvement in disease: Silver-Russell syndrome (SRS) ; Growth restriction, severe, with distinctive facies (GRDF)

Subcellular Location: Secreted

Protein Families: Insulin family

Tissue Specificity: Expressed in heart, placenta, lung, liver, muscle, kidney, tongue, limb, eye and pancreas.

Paythway: MAPKsignalingpathway

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P01344

Uniprot Entry Name: IGF2_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM