Recombinant Human Indian hedgehog protein (IHH), partial (Active) | CSB-AP000421HU

Recombinant Human Indian hedgehog protein (IHH) ,partial (Active) | CSB-AP000421HU | Cusabio

Protein Description: Partial

Alternative Name (s) : IHH,

Gene Names: IHH

Research Areas: Stem Cells

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 29-202aa

Sequence Info: II+GPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2 (CCL-226) cells is 3.0-10 μg/ml.

MW: 19.8 kDa

Purity: >96% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO) , to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) (By similarity) . {ECO:0000250}.

PubMed ID: 15489334; 7590746; 7720571; 9593755; 16335952; 20519495; 21537345; 11455389; 12384778; 12632327

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO) , to activate the transcription of target genes. Implicated in endochondral ossification

Involvement in disease: Brachydactyly A1 (BDA1) ; Acrocapitofemoral dysplasia (ACFD)

Subcellular Location: Indian hedgehog protein N-product: Cell membrane, Lipid-anchor, Extracellular side, Note=The N-terminal peptide remains associated with the cell surface, SUBCELLULAR LOCATION: Indian hedgehog protein C-product: Secreted, extracellular space

Protein Families: Hedgehog family

Tissue Specificity: Expressed in embryonic lung, and in adult kidney and liver.

Paythway: Hedgehogsignalingpathway

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 μm filtered 1 × PBS, pH 7.4

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q14623

Uniprot Entry Name: IHH_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM