Recombinant Human Gamma-crystallin D (CRYGD) | CSB-EP006020HU

Recombinant Human Gamma-crystallin D (CRYGD) | CSB-EP006020HU | Cusabio

Alternative Name(s): Gamma-D-crystallin Gamma-crystallin 4

Gene Names: CRYGD

Research Areas: Neuroscience

Organism: Homo sapiens (Human)

AA Sequence: GKITLYEDRGFQGRHYECSSDHPNLQPYLSRCNSARVDSGCWMLYEQPNYSGLQYFLRRGDYADHQQWMGLSDSVRSCRLIPHSGSHRIRLYEREDYRGQMIEFTEDCSCLQDRFRFNEIHSLNVLEGSWVLYELSNYRGRQYLLMPGDYRRYQDWGATNARVGSLRRVIDFS

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-174aa

Sequence Info: Full Length

MW: 47.6 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Crystallins are the dominant structural components of the vertebrate eye lens.

Reference: "Structural and evolutionary relationships among five members of the human gamma-crystallin gene family." Meakin S.O., Breitman M.L., Tsui L.-C. Mol. Cell. Biol. 5:1408-1414(1985)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Crystallins are the dominant structural components of the vertebrate eye lens.

Involvement in disease: Cataract 4, multiple types (CTRCT4)

Subcellular Location:

Protein Families: Beta/gamma-crystallin family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P07320

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM