Recombinant Human Desert hedgehog protein (DHH), partial (Active) | CSB-AP000411HU

Recombinant Human Desert hedgehog protein (DHH) ,partial (Active) | CSB-AP000411HU | Cusabio

Protein Description: Partial

Alternative Name (s) : DHH,

Gene Names: DHH

Research Areas: Stem Cells

Species: Homo sapiens (Human)

Source: E.Coli

Tag Info: Tag-Free

Expression Region: 24-198aa

Sequence Info: II+GPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2 (CCL-226) cells is 15-45 μg/ml.

MW: 19.9 kDa

Purity: >96% as determined by SDS-PAGE and HPLC.

Endotoxin: Less than 1.0 EU/µg as determined by LAL method.

Relevance: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

PubMed ID: 15489334; 11017805; 19561611; 20519495; 15356051

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Function: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

Involvement in disease: Partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD) ; 46,XY sex reversal 7 (SRXY7)

Subcellular Location: Desert hedgehog protein N-product: Cell membrane, Lipid-anchor, Extracellular side, Note=The N-terminal peptide remains associated with the cell surface, SUBCELLULAR LOCATION: Desert hedgehog protein C-product: Secreted, extracellular space

Protein Families: Hedgehog family

Tissue Specificity:

Paythway: Hedgehogsignalingpathway

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 μm filtered 10mM PB, pH 6.0, 300mM NaCl

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: O43323

Uniprot Entry Name: DHH_HUMAN

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM