Recombinant Human Arginase-1 (ARG1) (Active) | CSB-AP005561HU
- 5 to 10 Working Days
Recombinant Human Arginase-1 (ARG1) (Active) | CSB-AP005561HU | Cusabio
Protein Description: Full Length
Alternative Name (s) : Arginase-1; Liver-type arginase;Type I arginase;ARG1
Gene Names: ARG1
Research Areas: Signal Transduction
Species: Homo sapiens (Human)
Tag Info: C-terminal 6xHis-tagged
Expression Region: 1-322aa
Sequence Info: MSAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIGSISGHARVHPDLGVIWVDAHTDINTPLTTTSGNLHGQPVSFLLKELKGKIPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYILKTLGIKYFSMTEVDRLGIGKVMEETLSYLLGRKKRPIHLSFDVDGLDPSFTPATGTPVVGGLTYREGLYITEEIYKTGLLSGLDIMEVNPSLGKTPEEVTRTVNTAVAITLACFGLAREGNHKPIDYLNPPK
Biological Activity: Specific activity as determined by the production of urea during the hydrolysis of arginine is greater than 6000 pmol/min/µg.
MW: 35.8 kDa
Purity: Greater than 95% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU/µg as determined by LAL method.
Relevance: ARG1 is a member of the ureohydrolase family of enzymes. ARG1 can catalyze the hydrolysis of arginine to ornithine and urea. In the urea cycle, ARG1 catalyzes the fifth and final step, a series of biochemical reactions in mammals during which the body disposes of harmful ammonia. ARG1 is a cytosolic enzyme and expressed widely in the liver as part of the urea cycle. Inherited deficiency of this ARG1 causes argininemia, which is an autosomal recessive disorder characterized by hyperammonemia.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Function: Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.
Involvement in disease: Argininemia (ARGIN)
Subcellular Location: Cytoplasm, Cytoplasmic granule
Protein Families: Arginase family
Tissue Specificity: Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]) (PubMed:15546957) . Also detected in macrophages mycobacterial granulomas (PubMed:23749634) . Expressed in group2 innate lymphoid cells (ILC2s) during lung disease (PubMed:27043409) .
Buffer: 0.2 μm filtered 20 mM Tris-HCl, 150 mM NaCl, 20% Glycerol, 1 mM DTT, pH 7.4
Uniprot ID: P05089
Uniprot Entry Name:
HGNC Database Link: HGNC
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: OMIM