Recombinant Human Acid ceramidase (ASAH1) | CSB-EP619774HU

Recombinant Human Acid ceramidase (ASAH1) | CSB-EP619774HU | Cusabio

Alternative Name(s): Acylsphingosine deacylase N-acylsphingosine amidohydrolase Putative 32KDA heart protein Short name:PHP32

Gene Names: ASAH1

Research Areas: Signal Transduction

Organism: Homo sapiens (Human)

AA Sequence: QHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Source: E.coli

Tag Info: N-terminal 6xHis-SUMO-tagged

Expression Region: 22-395aa

Sequence Info: Full Length of Mature Protein

MW: 58.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.

Reference: "A new gene family predicted by a novel human heart cDNA."Churchill J.R., Wieland S.J., Hoffman S., Gallin E.K., Murphy P.M.Mol. Biol. Cell 6:418-418(1995)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.

Involvement in disease: Farber lipogranulomatosis (FRBRL); Spinal muscular atrophy with progressive myoclonic epilepsy (SMAPME)

Subcellular Location: Lysosome

Protein Families: Acid ceramidase family

Tissue Specificity: Broadly expressed with highest expression in heart.

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: Q13510

HGNC Database Link: HGNC

UniGene Database Link: UniGene

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: OMIM