Cusabio Mouse Recombinants
Recombinant Mouse Acid ceramidase (Asah1), partial | CSB-EP895296MO
- 3 - 7 Working Days
Recombinant Mouse Acid ceramidase (Asah1), partial | CSB-EP895296MO | Cusabio
Alternative Name(s): Acylsphingosine deacylase N-acylsphingosine amidohydrolase
Gene Names: Asah1
Research Areas: Signal Transduction
Organism: Mus musculus (Mouse)
AA Sequence: QAQDVPPWTEDCRKSTYPPSGPTYRGPVPWHTINLDLPPYKRWHELLAQKAPALRILVNSITSLVNTFVPSGKLMKMVDQKLPGMIGSLPDPFGEEMRGIADVTGIPLGEIISFNIFYELFTM
Tag Info: N-terminal 10XHis-GST-tagged and C-terminal Myc-tagged
Expression Region: 19-141aa
Sequence Info: Partial
MW: 43.8 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
Reference: "Cloning and characterization of the full-length cDNA and genomic sequences encoding murine acid ceramidase."Li C.-M., Hong S.-B., Kopal G., He X., Linke T., Hou W.-S., Koch J., Gatt S., Sandhoff K., Schuchman E.H.Genomics 50:267-274(1998)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
Involvement in disease:
Subcellular Location: Lysosome
Protein Families: Acid ceramidase family
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: Q9WV54
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A