Recombinant Escherichia coli Glucose-6-phosphate 1-dehydrogenase (zwf), partial | CSB-RP183744Ba

Recombinant Escherichia coli Glucose-6-phosphate 1-dehydrogenase (zwf), partial | CSB-RP183744Ba | Cusabio

Alternative Name(s): zwf; b1852; JW1841Glucose-6-phosphate 1-dehydrogenase; G6PD; EC 1.1.1.49) [Cleaved into: Extracellular death factor; EDF)]

Gene Names: zwf

Research Areas: Others

Organism: Escherichia coli (strain K12)

AA Sequence: MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTRIIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRLGAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEINDQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVGIEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDRSNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFYLRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPGLDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVDSITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRS

Source: E.coli

Tag Info: N-terminal GST-tagged

Expression Region: 1-486aa

Sequence Info: Partial

MW: 82 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.

Reference: A linear pentapeptide is a quorum-sensing factor required for mazEF-mediated cell death in Escherichia coli.Kolodkin-Gal I., Hazan R., Gaathon A., Carmeli S., Engelberg-Kulka H.Science 318:652-655(2007)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.

Involvement in disease:

Subcellular Location:

Protein Families: Glucose-6-phosphate dehydrogenase family

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P0AC53

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A