Recombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5) | CSB-YP317769CTL

Recombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5) | CSB-YP317769CTL | Cusabio

Alternative Name(s): URA5; CNG03730; Orotate phosphoribosyltransferase; OPRT; OPRTase; EC 2.4.2.10

Gene Names: URA5

Research Areas: Others

Organism: Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)

AA Sequence: MSSQALDSAKVAFIEAAIEHGVLLFGNFTLKSGRQSPYFFNAGLLYSSSLLSTTAQAYAKVLSSSRIPDFDVLFGPAYKGISLAAVSAVSLYQQTGKDIGYCYNRKEKKDHGEGGTMVGAPLKGRIVIIDDVLTSGKAIREAIDILKASPEAKLVGIVQLVDRQEKGQSGSGKSTVQEVEEEFGVPVEPIIGLDDIVKYLESSGKWEKELQEVRKYRAEYGVQRS

Source: Yeast

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Expression Region: 1-225aa

Sequence Info: Full Length

MW: 28.4 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Relevance: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).

Reference: "Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation." Edman J.C., Kwon-Chung K.J. Mol. Cell. Biol. 10:4538-4544(1990)

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).

Involvement in disease:

Subcellular Location:

Protein Families: Purine/pyrimidine phosphoribosyltransferase family, PyrE subfamily

Tissue Specificity:

Paythway:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Uniprot ID: P0CS95

HGNC Database Link: N/A

UniGene Database Link: N/A

KEGG Database Link: KEGG

STRING Database Link: STRING

OMIM Database Link: N/A