Recombinant Apis mellifera Venom serine carboxypeptidase | CSB-EP509855DNK
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Recombinant Apis mellifera Venom serine carboxypeptidase | CSB-EP509855DNK | Cusabio
Alternative Name(s): Allergen: Api m 9
Gene Names: N/A
Research Areas: Others
Organism: Apis mellifera (Honeybee)
AA Sequence: FTNVYPKPKYCPLLHEEDAGIPLFLTPLIENGKIDEARNKAVIQHKEVEAISSYAGFLTVNKKYNSNMFFWFFPALHDPKTAPVVLWLQGGPGATSMYGLFLENGPFIVTKNKTLKMREYSWNKCHNLLYIDNPVGTGFSFTEDERGYATNETHVGRDVHTALVQFFELFPELQTNDFYVTGESYGGKYVPAVSHAIKDYNIKAKIKINLKGLAIGNGLTDPVNQLDYGDYLYQLGLLDANGRNLFQKYEEQGKNLIKQEKWLEAFDLFDELLDGDITQQPSLYKNLTGFDYYFNYLHEKDPSNDSDYMVEWLQRADVRKAIHVGNRTFIPESKKVEKYMKADVMQSLAVLIADLTQHYRVLIYNGQLDIIVAYPLTENYLQKLKWPGAEKYKTAQRKVWFVGNELAGYSKTVDSLTEVLVRNAGHMVPLDQPKWALDLITRFTHNKGF
Tag Info: N-terminal 6xHis-SUMO-tagged
Expression Region: 19-467aa
Sequence Info: Full Length of Mature Protein
MW: 67.6 kDa
Purity: Greater than 90% as determined by SDS-PAGE.
Relevance: Release of a C-terminal amino acid with broad specificity.
Reference: "Identification, recombinant expression and characterization of high molecular weight hymenoptera venom allergens."Blank S., Seismann H., Braren I., Bockisch B., Bredehorst R., Ollert M., Grunwald T., Spillner E.Submitted (FEB-2009)
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Involvement in disease:
Subcellular Location: Secreted
Protein Families: Peptidase S10 family
Tissue Specificity: Expressed by the venom duct.
Form: Liquid or Lyophilized powder
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Uniprot ID: C9WMM5
HGNC Database Link: N/A
UniGene Database Link: UniGene
KEGG Database Link: KEGG
STRING Database Link: STRING
OMIM Database Link: N/A